VivaScope® 2500 Multilaser

The VivaScope® 2500 makes it possible for the first time to subject large specimens of freshly sampled tissues to pathological analyses at cellular resolution and in real time. Skin and other tissue samples are depicted without the traditional preparation procedures usually required. The VivaScope® 2500 Multilaser uses two lasers with wavelengths of 658 nm (red) and 488 nm (blue) in addition to the infrared laser with wavelength of 830 nm integrated into the standard in vivo VivaScope® devices.

VivaScope® 2500 Multilaser:
Confocal laser scanning microscopy for ex vivo use

Medical problems sometimes require differentiated methods. This is why the VivaScope® 2500 Multilaser for ex vivo applications was developed. The VivaScope® 2500 Multilaser uses two lasers with wavelengths of 658 nm (red) and 488 nm (blue) in addition to the infrared laser with wavelength of 830 nm integrated into the standard in vivo VivaScope® devices. A fluorescent dye may be applied to the tissue to be examined prior to using the VivaScope®. The dye is excited by the respective laser light. The emitted fluorescence produces a contrast during the imaging process that helps to display the histological structure due to the dye distribution. The generated image is characterized not only by the cell morphology but also by the direct properties of the fluorophore.

Users of the VivaScope® 2500 Multilaser can apply all three lasers together or each laser individually for the same tissue sample and switch between all three as needed at any time. Standard filters for the following fluorophores are included in the delivery scope: Methylene blue and toluidine blue (red laser), as well as acridine orange (blue laser). When performing Mohs surgeries, the physician may benefit from these additional options, especially concerning the precise detection of margin layers between tumors and healthy tissue, as indicated in the studies which have been published so far.

VivaScope® 2500 Multilaser Technical Data

Optical section thickness: ≤ sections that are microtomed for routine histology
Imaging depth: up to 200 µm (depends on tissue type)
Viewable section, individual image: 750 µm x 750 µm (usable screen section)
Displayed image field: +/- 8.0 mm in direction of x and y (16-mm²)
Image resolution: 1000 x 1000 pixels (Nyquist-optimized)
Magnification: 400x
Frame rate: 9 frames per second
Monitor: 19", 1280 x 1024 pixels, color flat panel display
Software: VivaScan®
Optical operating power: red and infrared laser: CDRH Class 1, EU Class 2 (for Class 2 lasers, viewing the laser output with certain optical instruments within 100 mm may pose an eye hazard); blue laser: to be determined, therefore eye protection may be recommended.
Imaging wavelength: 830 nm, 658 nm, 488 nm
Imaging head: Benchtop, inverted microscope setup
Included objective: Lucid StableViewTM F.L. = 5.3 mm, 0.9 NA water immersion
Operating temperature range: 55°F to 85°F (13°C to 30°C)
Power source: 110-230 VAC 50-60 Hz
Operating humidity: non-condensing
Certifications: FCC Class A, EC